Circulating miRNA-770-5p and miRNA-30d-5p as potential biomarkers in vasoplegic syndrome after on-pump coronary artery bypass surgery – PREVENT trial

Abstract Introduction Vasoplegic syndrome (VS) is one of the most common unexpected complications following cardiothoracic surgery, approaching a 25% mortality rate. No standardized methods for diagnosing VS are available. A biomarker valuable tool in all fields medicine, especially cardiovascular disease when patient has to undergo invasive surgery as on-pump CABG. MicroRNAs (miRNAs) have been studied and employed biomarkers numerous diseases, however, there no studies regarding their expression VS. Purpose To discover new predictor by comparing miRNA profiles from patients who evolved versus those did not evolve after Coronary artery bypass graft (CABG). Methods nested case-control study had an exploratory nature involved initial cohort 87 underwent CABG elective or urgency procedures,considering low surgical risk (STS score <2%). For this analysis, we compared 30 patients, divided into two groups: (VASO group, n=15) (NONVASO surgery. perform profiling, target prediction, identify putative targets dysregulated miRNAs, whole blood samples were collected anesthetic induction before incision chest (Figure 1A). Results We identified among 754 screened eight differentially circulating miRNAs VASO NONVASO groups 1B). Six increased (hsa-miR-548c-3p, hsa-miR-30d-5p, hsa-miR8 199b-5p, hsa-miR-183-3p, hsa-miR-571, hsa-miR-383-5p) decreased (hsa-miR-1236-3p, hsa-miR-770-5p) hsa-miR-1236-3p was statistically significant 1C). The ROC curves each single yielded top 2 highest AUC values 0.8333 0.8178 hsa-miR-770-5p respectively. combination these value 0.9615 with 84.6% sensitivity 91.67% specificity distinguishing showed superior diagnostic power that 2). Computational analyses enriched pathway “Apelin Liver Signaling Pathway” 14 out 26 molecules within (53,8%) containing higher number miRNAs. There statistical difference preoperative, postoperative, EuroSCORE II, variables both groups. Conclusion(s) miRNA-770-5p miRNA-30d-5p could be potential VS, strategy diagnosis since distinguish disease. capability predicting high accuracy would drastically change clinical management patient's referral cardiac helping decision-making once risks proved unable predict low-risk patients. Funding Acknowledgement Type funding sources: Foundation. Main source(s): Fundação de Amparo Pesquisa São Paulo - FAPESP